Glossary · 2026-09-24
By Alice Kay · Last reviewed 24 Sep 2026
Chromatogram on a peptide COA
Research-use notice: This article summarizes published scientific literature for educational purposes. It is not medical advice and does not provide dosing, administration or human-use information. Findings described in the literature do not apply to any BlueNex Labs product, which is supplied only for in-vitro laboratory research and not for human or animal use.
A chromatogram is the picture of a chromatographic run: detector signal against time. Peaks are integrated, and a peptide Certificate of Analysis often reduces that picture to an area percent for the named peak. The percentage belongs to the stated method. It does not, by itself, name the sequence that produced the peak.
Read the trace next to the mass result, not instead of it. Method names such as HPLC or UPLC stay on the PDF. For the comparison note, see HPLC versus LC-MS. A photograph of a chromatogram is not the laboratory file. Research use only.
Published lot files for this catalogue are indexed in the certificates library. This definition does not add a report, and it does not assign a task identifier. Research use only.
Frequently asked questions
- Is a chromatogram the same record as a mass spectrum?
- No. A chromatogram is signal versus time. A mass spectrum is ion signal versus mass-to-charge. Some reports include both. Some include only one.
- Does a single tall peak prove identity?
- No. Peak shape supports a purity discussion under that method. Identity still needs the mass comparison the report actually prints.
Research use only. BlueNex Labs does not provide medical or veterinary advice. Confirm your institution’s policies before purchase. How a comparison or class note is checked sits on documentation standards. Articles are written and reviewed by Alice Kay, Ph.D.