HPLC on a peptide Certificate of Analysis

HPLC means high-performance liquid chromatography. A dissolved sample is pushed through a column, and a detector, often ultraviolet, records peaks over time. On a peptide Certificate of Analysis the useful figure is usually an area percent: the named peak's area divided by the total integrated area under the stated method. That percentage describes separation under those conditions. It does not, by itself, prove which sequence produced the peak.

BlueNex Labs lot reports that cite HPLC or the related UPLC method should be read next to the mass result. For the longer comparison, see HPLC versus LC-MS. A purity figure for one batch does not document a later fill or a different milligram size.

What the area percent is measuring

HPLC, high-performance liquid chromatography, pushes a dissolved sample through a column and records detector response over time. On a peptide Certificate of Analysis the figure laboratories usually quote is an area percent: the named peak's area divided by the integrated area under the stated method. The percentage describes that separation. It depends on the column, the gradient, the wavelength, and which peaks the analyst included. It does not, by itself, name the sequence. Identity is the mass result on the same file, or on a paired file, compared with the mass calculated from the stated residues.

UPLC is the same idea at higher pressure. Do not average an HPLC percentage with a UPLC percentage from a different lot. Record the method name printed on the PDF you opened. For the longer comparison, see how peptide purity is measured. A cropped image that shows one percentage and hides the sample name is not the report. Open the file.

How to file an HPLC line against a live SKU

Write the percentage beside the method, the sample name, the date, and the milligram fill. For BPC-157 10 mg, see the BPC-157 10 mg certificate. GHK-Cu 50 mg has mass and endotoxin links at the GHK-Cu 50 mg certificate row. Retatrutide 10 mg, 20 mg, and 30 mg are separate listings: a 10 mg chromatogram does not document a 20 mg or 30 mg vial. Cap colour, when the library prints it, is part of the match. A blend such as BPC-157 / TB-500 5 mg/5mg has its own row. That area percent does not document TB-500 10 mg, which publishes its purity file on the TB-500 10 mg certificate row.

Endotoxin is not an HPLC peak. If the library row includes an endotoxin file, copy the number and the unit. If it does not, write that the test was not published. An HPLC percentage does not imply that line. For the reading guide, see how to read a peptide COA. The index is the certificates library.

What a high percentage does not authorize

A high area percent is not a Drug Identification Number and it is not a Health Canada authorization. Research-use-only labelling stays in force whether the peak is narrow or crowded. A crowded chromatogram with a plausible mass is an impure sample of a plausible sequence. A narrow peak on the wrong mass is a different compound. Read both lines before the vial enters a study file. This entry does not rank laboratories and it does not set a personal-use concentration. Canada fulfilment, if you are ordering the vial this chromatogram belongs to, is Interac e-Transfer and Canada Post Xpresspost, described on the procurement guide.

Retention time is part of the same chromatogram and it is not a second purity figure. Record it when the PDF prints it, next to the method name, because a later lot on a different column can move. For the glossary line, see retention time. Store the sealed vial the day it arrives, using the storage guide, and keep the PDF with the lot identifier you will write on that record. A chromatogram filed in a drawer does not help if the vial is on a sunny shelf. Research use only.