Does an HPLC percentage identify the peptide?

No. HPLC reports how the sample separated and is commonly used for a purity percentage. Identity is usually the mass result on the same report, compared with the mass calculated from the sequence.

Is HPLC the same number as an endotoxin result?

No. Endotoxin is a separate assay, often LAL or recombinant Factor C, reported in endotoxin units. An HPLC area percent does not imply an endotoxin value.

Glossary · 2026-09-24

Scientific lead: Alice Kay, Ph.D.

HPLC on a peptide Certificate of Analysis

HPLC means high-performance liquid chromatography. A dissolved sample is pushed through a column, and a detector, often ultraviolet, records peaks over time. On a peptide Certificate of Analysis the useful figure is usually an area percent: the named peak's area divided by the total integrated area under the stated method. That percentage describes separation under those conditions. It does not, by itself, prove which sequence produced the peak.

BlueNex Labs lot reports that cite HPLC or the related UPLC method should be read next to the mass result. The longer comparison is HPLC versus LC-MS. A purity figure for one batch does not document a later fill or a different milligram size.

Frequently asked questions

Does an HPLC percentage identify the peptide?: No. HPLC reports how the sample separated and is commonly used for a purity percentage. Identity is usually the mass result on the same report, compared with the mass calculated from the sequence.
Is HPLC the same number as an endotoxin result?: No. Endotoxin is a separate assay, often LAL or recombinant Factor C, reported in endotoxin units. An HPLC area percent does not imply an endotoxin value.

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